Phenotypic diversity of Flo protein family-mediated adhesion inSaccharomyces cerevisiae

Mutationsatdi¡erent sites inmembersoftheGpr1/Fun34/YaaH protein familycausehypersensitivity toacetic acid inSaccharomyces cerevisiae aswell as inYarrowia lipolytica

protein familycausehypersensitivity toacetic acid inSaccharomyces cerevisiae aswell as inYarrowia lipolytica Marcus Gentsch, Margret Kuschel, Susan Schlegel & Gerold Barth Department of Pediatrics, University Clinic Carl Gustav Carus, Dresden, Germany; Department of Radiotherapy and Radiation Oncology, University Clinic Carl Gustav Carus, Dresden, Germany; Institute of Biochemistry and Molecula...

Protein mediated membrane adhesion

Many Saccharomyces cerevisiae Cell Wall Protein Encoding Genes Are Coregulated by Mss11, but Cellular Adhesion Phenotypes Appear Only Flo Protein Dependent

The outer cell wall of the yeast Saccharomyces cerevisiae serves as the interface with the surrounding environment and directly affects cell-cell and cell-surface interactions. Many of these interactions are facilitated by specific adhesins that belong to the Flo protein family. Flo mannoproteins have been implicated in phenotypes such as flocculation, substrate adhesion, biofilm formation, and...

Prions, protein homeostasis, and phenotypic diversity.

Prions are fascinating but often misunderstood protein aggregation phenomena. The traditional association of the mammalian prion protein with disease has overshadowed a potentially more interesting attribute of prions: their ability to create protein-based molecular memories. In fungi, prions alter the relationship between genotype and phenotype in a heritable way that diversifies clonal popula...

Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae.

Large-scale protein overexpression phenotype screens provide an important complement to the more common gene knockout screens. Here, we have targeted the so far poorly understood Saccharomyces cerevisiae membrane proteome and report growth phenotypes for a strain collection overexpressing approximately 600 C-terminally tagged integral membrane proteins grown both under normal and three differen...